Ultrastructural impairment of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice

AIM: To investigate the ultrastructural changes of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice. METHODS: BALB/c mice were randomly divided into diabetic group and control group. The expression of insulin and platelet-endothelial cell adhesion molecule-1 (CD31) in islet microvessels was detected by immunohistochemical staining. The ultrastructural changes of islet β cells and islet microvessels were observed under transmission electron microscope. RESULTS: Compared with control group, the number of islet β cells, ratio of β cells/α cells, average number of secretory granules in β cells and insulin expression area per islet in diabetic group were significantly decreased (P<0.01). Besides, diabetic group had fewer microvessels with lower expression of CD31 (P<0.01). Mitochondria in islet microvascular endothelial cells and pericytes in diabetic group were swelling. The basement membrane of islet microvessels became thicker in diabetic group (P<0.01). CONCLUSION: Islet microvascular endothelial cells were impaired in type 1 diabetic mice.     

我国城市蔬菜价格波动的实证分析

为了研究我国城市蔬菜价格波动趋势,了解影响蔬菜价格波动的主要因素,对全国36个大中城市居民的鲜菜消费价格指数进行分析,并选取大白菜、番茄、黄瓜、茄子、青椒5个蔬菜品种进行重点考察,利用X-12季节调整模型,将蔬菜价格的季节性因素剥离,运用非对称误差修正模型(APT-ECM)模型验证蔬菜价格的非对称传导,从产业链价格传导视角研究这5种蔬菜的集贸市场价格波动。结果表明:2009—2014年,我国36个大中城市居民鲜菜消费价格指数总体呈上涨趋势,从95.6增长到134.8,年均增长7.1%;秋冬季节,蔬菜供给量全年最低,蔬菜价格上涨到最高水平;从产业链价格传递视角来看,全国蔬菜批发市场价格与集贸市场价格之间的价格传递具有非对称性,批发市场价格的波动将会导致下游集贸市场价格更大幅度的波动;蔬菜生产成本的推动、蔬菜生产的季节性、政府的宏观调控等是引起我国城市蔬菜价格波动出现以上特征的主要影响因素。     

Establishment and Optimization of INH Epitope Peptide Vaccine for Detection of Antibody

In this study, an indirect ELISA method was established to detect inhibin hormone (INH) epitope peptide vaccine antibody, it would provide oretical reference for the determination of Fine-wool sheep after active immune body INH epitope peptide vaccine antibody. On the basis of the predecessors, using indirect ELISA method to determinate serum INH epitope peptide antibody levels of sheep, and through control different experimental conditions to look for the best experimental conditions. Through explorating the experimental conditions, finally, the testing experiment conditions were determined, which was blocked solution with skimmed milk powder, INH and GnIH synthetic peptides dilution degrees for 20 000 times, the optimum reaction time was 60 min, the best color action time was 15 min. In this experiment, a kind of method to detection antibody in the body after INH active immune sheep was built, it would provide a reference for future research.     

Effects of Different Nitrogen Treatments on N use Efficiency and Grain Yield of Mechanically-transplanted Hybrid Rice

In order to investigate effects of topdressing on grain yield and N use efficiency of mechanically-transplanted hybrid rice,this experiment was conducted with 2-line hybrid rice cultivars,Quanliangyou 1 and Quanliangyou 681 with 3 application levels of N fertilizer,No,N1,N2,respectively.The results showed that there were no significant differences in grain yields of the 2 varieties between treatments N1 and N2.It was indicated that N is not the factor which could limit grain yield within limited application levels of N fertilizer.High N fertilizer treatment could contribute to the formation of effective panicles and spikelets per panicle,but it could also lead to the decline in grain filling rate.It was suggested that lowering the amount of Nfertilizer could facilitate improvement of N-use efficiency.     

浙西南茶园土壤酸化成因及修复治理

茶业是浙西南比较优势较强的一个农业产业。纵观近30年来,土地为茶产业提供巨大财富的同时,也遭受了土壤酸化、养分失衡等因素的影响。本文分析了近30年松阳茶园土壤酸化状况,针对酸化原因提出了修复治理措施,服务于浙西南茶产业转型升级。     

Heavy Metals Residues in Soil,Feed and Beef of the Cattle Industry in Yili area of Xinjiang

In order to understand the situation of heavy metal residues of the beef industry chain in Yili area of Xinjiang.The heavy metals (Pb, As, Hg, Cr and Cd) in soil, feed and beef of the cattle farm were determined using the atomic absorption spectrometry.The results showed that the heavy metal (Pb, As, Hg, Cr and Cd)contents in soil samples were 0.03-0.20, 3.87-8.30, 0.15-0.23, 0.09-0.48 and 0.41-0.86 mg/kg, respectively.The heavy metal (Pb, As, Hg, Cr and Cd) contents in feed samples were 3.01-18.09, 75.35-94.27, 19.65-24.37, 0.06-1.04 and 1.81-8.46 μg/kg, respectively.The heavy metal (Pb, As, Hg, Cr and Cd) contents in beef samples were 0.23-0.54, 1.22-7.12, 0.28-0.53, 0.01-0.27 and 0.02-0.03 μg/kg, respectively.The correlation coefficient of the heavy metal element between soil and feed, feed and beef, soil and beef were 0.96, 0.99 and 0.98, respectively.It was concluded that the heavy metal (Pb, As, Hg, Cr and Cd) contents in soil, feed and beef of the cattle farms in Yili area did not exceed the standard and that in the soil, feed and beef had the high correlations.     

冬油菜区试品种性状分析

[目的]分析冬油菜区试品种性状。[方法]对2013—2016年度参加国家冬油菜品种区域试验的长江上游、中游、下游、黄淮及早熟5个区组共539个油菜新品种的产量和产油量、产量构成因素、品质性状及抗病性进行了分析。[结果]2013—2016年度国家冬油菜新品种区域试验3年平均产量为2 718.6 kg/hm~2,比2012年(不含早熟组)增加93.4 kg/hm~2,增加了3.56%,3年平均产油量为1 169.1 kg/hm~2,比2012年(不含早熟组)增加58.2 kg/hm~2,增加了5.24%;产量构成因素中,单株有效角果数和角粒数不同区组之间差异较大、但不同年份之间差异不大,千粒重在不同年份和不同区组之间变化不大;品质性状中,芥酸和硫苷含量完全符合国家低芥酸和低硫苷标准,含油量不同年份间总体呈逐年增加趋势,2015—2016年度平均为43.49%,比2012年增加了1.41%;菌核病和病毒病的发病指数不同区组之间差异明显,长江下游组均最高,不同年份间也波动明显,表明环境条件对油菜抗逆性也有显著影响。[结论]该研究为当地油菜产业发展和育种工作提供参考。     

中国不同品种谷子抗性淀粉分布规律

抗性淀粉是谷子中的重要功能成分。对来自中国4个生态区10个省份216个谷子品种抗性淀粉含量进行分析。结果表明,中国谷子地方品种抗性淀粉平均值为2.43%,含量变幅为0.00%~6.74%,变异系数为50.26%,呈偏正态分布;不同生态区谷子抗性淀粉含量从高到低依次为内蒙古高原、华北平原、东北平原、黄土高原,且内蒙古高原和黄土高原之间差异显著(P0.05);不同省份抗性淀粉含量存在一定差异,其中黑龙江谷子抗性淀粉含量显著高于内蒙古和甘肃(P0.05),其他省份间差异不大;不同省份谷子(黑龙江除外)和不同生态区品种谷子抗性淀粉含量以2.00%~4.00%为主。     

长期施肥对南方典型水稻土养分含量及真菌群落的影响

利用江西省农业科学院31年的长期肥料定位试验,选取不施肥(对照)、单施化肥、70%化肥配施30%有机肥、50%化肥配施50%有机肥和30%化肥配施70%有机肥等5个处理,通过常规分析和454-高通量测序技术,研究了长期不同施肥条件下,我国南方典型水稻土养分含量和真菌群落结构的变化。结果表明,在酸性水稻土上,长期单施化肥显著降低土壤p H值,但随着有机肥配施比例的提高p H明显上升;有机和无机肥配施显著提高土壤有机质、有效氮磷含量以及微生物碳氮量。单施化肥土壤真菌18S r DNA序列数比配施有机肥的多1倍,但真菌种(属)数减少了11~40种;前20种优势真菌的丰富度占真菌总量的78.82%~91.51%,以子囊菌最多(7?13种),所占比例最大(23.13%?75.09%);与对照相比,配施有机肥的土壤中有14?15种优势真菌与之相同,而单施化肥的土壤中仅有10种一致;主成分分析结果表明单施化肥处理的真菌群落组成与其他各处理存在显著差异。因此,单施化肥造成土壤酸化加剧,真菌数量成倍增加,但种类显著减少,其丰富度和多样性明显降低,并改变优势真菌种群,相应提高了土壤病原真菌过度繁殖的风险。而有机和无机肥配施有利于维持水稻土壤健康生态环境和真菌种群多样性。     

辣椒新品种‘苏椒25号’

‘苏椒25号’为早熟辣椒杂交一代新品种。果实长灯笼形,纵径13.5 cm,果肩横径4.5 cm,果肉厚度0.30 cm,单果质量62.1 g,果面微皱有光泽,青果绿色,成熟果红色,味微辣,鲜果维生素C含量1.15 mg·g~(-1),产量为41.0 t·hm~(-2)左右。适合在江淮、黄淮流域保护地栽培。